Search for: [Abstrakt = "The doctoral dissertation is devoted to the computer\-aided design of fluorescent probes with two\-photon absorption properties tailored for bioimaging applications. The dissertation encompasses two objectives\: i\) the design of novel probes with significant two\-photon absorption strengths, and ii\) the development of entirely non\-empirical computational protocol for robust and cost\-effective simulations of vibrationally\-resolved absorption spectra. Organic dyes containing tetra\-coordinated boron atom were studied in this thesis on account of their good photophysical properties."]