Title:

Membranes affinity of hydroxycamptothecins, anticancer agents, determined by fluorescence spectra analysis

Group publication title:

Optica Applicata

Creator:

Cyrankiewicz, Michał ; Ziomkowska, Blanka ; Kruszewski, Stefan

Contributor:

Gaj, Miron. Redakcja ; Wilk, Ireneusz. Redakcja

Subject and Keywords:

optyka ; 0-hydroxycamptothecin ; SN-38 ; DB-67 ; fluorescence ; liposomes

Description:

Optica Applicata, Vol. 36, 2006, nr 2-3, s. 209-215

Abstrakt:

In this paper the method of determination of membranes affinity of hydroxycamptothecins is described. Under physiological conditions hydroxycamptothecins easily hydrolyze and convert into inactive carboxylate form. The process of deactivation is inhibited when the molecules of drug are bound to cell membranes so it is desirable that hydroxycamptothecins molecules bind easily to membranes. A quantitative measure of drugs affinity to membranes is the association constant. To determine this parameter the small unilamellar liposomes are used as model membranes. The affinities of 10-hydroxycamptothecin, SN-38 and DB-67 to membranes are determined. The association constants are calculated on the basis of changes of fluorescence spectra.

Publisher:

Oficyna Wydawnicza Politechniki Wrocławskiej

Place of publication:

Wrocław

Date:

2006

Resource Type:

artykuł

Source:

<sygn. PWr A3481II> ; click here to follow the link ; click here to follow the link

Language:

eng

Relation:

Optica Applicata ; Optica Applicata, Vol. 36, 2006 ; Optica Applicata, Vol. 36, 2006, nr 2-3 ; Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki

Rights:

Wszystkie prawa zastrzeżone (Copyright)

Access Rights:

Dla wszystkich w zakresie dozwolonego użytku

Location:

Politechnika Wrocławska